Journal: Journal of Orthopaedic Surgery and Research
Article Title: B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts
doi: 10.1186/s13018-024-05414-3
Figure Lengend Snippet: Effects of B-nHAp on genes related to cell proliferation and differentiation. A MRNA expressions of CCND1 , MYC , and PCNA, marker molecules of cell proliferation determined through RT-qPCR, B MRNA expressions of ALPL and RUNX2, marker molecules of cell differentiation, measured via RT-qPCR, and C marker molecules of cell proliferation and differentiation at the protein expression level determined by Western blotting. * P < 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: Then, primary antibodies against CCND1 , MYC , PCNA , ALPL , and RUNX2 (1: 1000, Wuhan Boster Biological Technology Co., Ltd., Cat. No. AF1011, AF0511, AF0921, AF0531, AF0591, respectively) and secondary antibodies (goat anti-rabbit IgG conjugated with HRP, 1:5000, Wuhan Boster Biological Technology Co., Ltd., Cat. No. BA1054) were added for membrane incubation, followed by color development with ECL reagent (TransGen Biotech Co., Ltd., Beijing, Cat. No. SQ201).
Techniques: Marker, Quantitative RT-PCR, Cell Differentiation, Expressing, Western Blot